Genotyping and primer details


Genotyping and primer details for 629D03 - At3g22220
Line ID 629D03
Locus At3g22220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:7841770 of line 629D03 Chr3:7841770
   
Insertion allele amplicon 8474 - 28lx
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 28lx: 5'-CTGTCGAAAGATGTTTAAAGGAGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:7842248
   
wt amplicon (over InsPos) 04tl - 28lx
wt amplicon start Chr3:7841355
wt amplicon end Chr3:7842248
wt amplicon size 894 bp
Locus specific primer 28lx: 5'-CTGTCGAAAGATGTTTAAAGGAGG-3'
2nd locus specific primer 04tl: 5'-GCGTAATGATCTTCACATTTGGTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11