Genotyping and primer details


Genotyping and primer details for 631E03 - At2g47320
Line ID 631E03
Locus At2g47320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:19428804 of line 631E03 Chr2:19428804
   
Insertion allele amplicon 8474 - NK62
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NK62: 5'-AACCAAGCTTATCCAAACAAGAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:19429373
   
wt amplicon (over InsPos) JK32 - NK62
wt amplicon start Chr2:19428416
wt amplicon end Chr2:19429373
wt amplicon size 957 bp
Locus specific primer NK62: 5'-AACCAAGCTTATCCAAACAAGAGA-3'
2nd locus specific primer JK32: 5'-TGTAAAGCATTTTGTGATTCAAGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11