Genotyping and primer details


Genotyping and primer details for 632G08 - At2g35640
Line ID 632G08
Locus At2g35640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:14982872 of line 632G08 Chr2:14982872
   
Insertion allele amplicon 8474 - 27sf
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 27sf: 5'-CCTTCCCACTAAATATAACGACCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:14982286
   
wt amplicon (over InsPos) 34tf - 27sf
wt amplicon start Chr2:14982286
wt amplicon end Chr2:14983205
wt amplicon size 920 bp
Locus specific primer 27sf: 5'-CCTTCCCACTAAATATAACGACCA-3'
2nd locus specific primer 34tf: 5'-TTCTCTTTCCTCTCAGTCTTGTCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11