Genotyping and primer details


Genotyping and primer details for 634F06 - At2g04050
Line ID 634F06
Locus At2g04050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:1339011 of line 634F06 Chr2:1339011
   
Insertion allele amplicon 8474 - KS55
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KS55: 5'-GCTGGAGTACCTCACATAACATGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:1338484
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11