Genotyping and primer details


Genotyping and primer details for 634G12 - At3g45410
Line ID 634G12
Locus At3g45410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:16655233 of line 634G12 Chr3:16655233
   
Insertion allele amplicon 8474 - EU25
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EU25: 5'-TCCATTGATCTCTCTCATGCATAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:16655692
   
Insertion allele amplicon 8474 - TH45
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer TH45: 5'-GAACCAAGTTCCGATGTTGTAAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:16654816
   
wt amplicon (over InsPos) TH45 - EU25
wt amplicon start Chr3:16654816
wt amplicon end Chr3:16655692
wt amplicon size 877 bp
Locus specific primer EU25: 5'-TCCATTGATCTCTCTCATGCATAC-3'
2nd locus specific primer TH45: 5'-GAACCAAGTTCCGATGTTGTAAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11