Genotyping and primer details


Genotyping and primer details for 635H12 - At3g56520
Line ID 635H12
Locus At3g56520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:20947229 of line 635H12 Chr3:20947229
   
Insertion allele amplicon 8474 - 21lu
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 21lu: 5'-CTTTAATCATCCTGTGAAAGAGCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:20947519
   
wt amplicon (over InsPos) 08mu - 21lu
wt amplicon start Chr3:20946694
wt amplicon end Chr3:20947519
wt amplicon size 826 bp
Locus specific primer 21lu: 5'-CTTTAATCATCCTGTGAAAGAGCC-3'
2nd locus specific primer 08mu: 5'-AATCCGGTTCTTGTAACGATAATG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11