Genotyping and primer details


Genotyping and primer details for 637G05 - At2g44440
Line ID 637G05
Locus At2g44440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:18337917 of line 637G05 Chr2:18337917
   
Insertion allele amplicon 8474 - HP43
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer HP43: 5'-GCCGTGTTAACTCAGATGATACAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:18338274
   
wt amplicon (over InsPos) 02fa - HP43
wt amplicon start Chr2:18337561
wt amplicon end Chr2:18338274
wt amplicon size 714 bp
Locus specific primer HP43: 5'-GCCGTGTTAACTCAGATGATACAA-3'
2nd locus specific primer 02fa: 5'-AAAAACTGAAACTGAATGCCAACT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11