Genotyping and primer details


Genotyping and primer details for 645G04 - At1g05780
Line ID 645G04
Locus At1g05780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:1727257 of line 645G04 Chr1:1727257
   
Insertion allele amplicon 8474 - 42sj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 42sj: 5'-TGAATGTGAAGATCATCAAAAAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:1727828
   
wt amplicon (over InsPos) 33tf - 42sj
wt amplicon start Chr1:1726887
wt amplicon end Chr1:1727828
wt amplicon size 942 bp
Locus specific primer 42sj: 5'-TGAATGTGAAGATCATCAAAAAGC-3'
2nd locus specific primer 33tf: 5'-CTCTCCAATTTTCTTTTGGTTGAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11