Genotyping and primer details


Genotyping and primer details for 646F05 - At4g34220
Line ID 646F05
Locus At4g34220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:16383546 of line 646F05 Chr4:16383546
   
Insertion allele amplicon 8474 - CS65
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CS65: 5'-CAAAGGGTTTATTGGAGCTGTACT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:16383089
   
wt amplicon (over InsPos) 08bv - CS65
wt amplicon start Chr4:16383089
wt amplicon end Chr4:16383835
wt amplicon size 747 bp
Locus specific primer CS65: 5'-CAAAGGGTTTATTGGAGCTGTACT-3'
2nd locus specific primer 08bv: 5'-AACACACCAGACATGTTCAGAGTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11