Genotyping and primer details


Genotyping and primer details for 651F05 - At1g77660
Line ID 651F05
Locus At1g77660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:29186333 of line 651F05 Chr1:29186333
   
Insertion allele amplicon 8474 - 55by
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 55by: 5'-CATGGTTTTGGAGTTTACTGGTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:29186786
   
wt amplicon (over InsPos) 04dg - 55by
wt amplicon start Chr1:29185778
wt amplicon end Chr1:29186786
wt amplicon size 1009 bp
Locus specific primer 55by: 5'-CATGGTTTTGGAGTTTACTGGTTT-3'
2nd locus specific primer 04dg: 5'-TACAAAACGAATCACATATTCCGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11