Genotyping and primer details


Genotyping and primer details for 651G11 - At4g24610
Line ID 651G11
Locus At4g24610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:12702551 of line 651G11 Chr4:12702551
   
Insertion allele amplicon 8474 - 20fx
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 20fx: 5'-AACTTCTCAGCAAAACAAAACGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:12701902
   
wt amplicon (over InsPos) 13gs - 20fx
wt amplicon start Chr4:12701902
wt amplicon end Chr4:12703151
wt amplicon size 1250 bp
Locus specific primer 20fx: 5'-AACTTCTCAGCAAAACAAAACGAT-3'
2nd locus specific primer 13gs: 5'-GGTAGCAACTTCTTTTTCTTCCCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11