Genotyping and primer details


Genotyping and primer details for 652D01 - At2g19120
Line ID 652D01
Locus At2g19120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:8287753 of line 652D01 Chr2:8287753
   
Insertion allele amplicon 8474 - 02um
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 02um: 5'-GCTGCAGCTTTTGCTACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:8288378
   
wt amplicon (over InsPos) 34vc - 02um
wt amplicon start Chr2:8287321
wt amplicon end Chr2:8288378
wt amplicon size 1058 bp
Locus specific primer 02um: 5'-GCTGCAGCTTTTGCTACA-3'
2nd locus specific primer 34vc: 5'-GCGAGCAGAGAGATAGAGAGAAAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11