Genotyping and primer details


Genotyping and primer details for 655G04 - At5g40350
Line ID 655G04
Locus At5g40350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:16139692 of line 655G04 Chr5:16139692
   
Insertion allele amplicon 8474 - JV41
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer JV41: 5'-CCGAAAGATCCGTAAATAAATCAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:16139385
   
wt amplicon (over InsPos) 12au - JV41
wt amplicon start Chr5:16139385
wt amplicon end Chr5:16140275
wt amplicon size 891 bp
Locus specific primer JV41: 5'-CCGAAAGATCCGTAAATAAATCAA-3'
2nd locus specific primer 12au: 5'-CTCTCTGGTTAGAATTGTTACGGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11