Genotyping and primer details


Genotyping and primer details for 658A12 - At5g57150
Line ID 658A12
Locus At5g57150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:23154529 of line 658A12 Chr5:23154529
   
Insertion allele amplicon 8474 - 27uv
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 27uv: 5'-ACATTTATTGGGTTGTGTGGTTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:23154166
   
wt amplicon (over InsPos) 70wa - 27uv
wt amplicon start Chr5:23154166
wt amplicon end Chr5:23155468
wt amplicon size 1303 bp
Locus specific primer 27uv: 5'-ACATTTATTGGGTTGTGTGGTTTT-3'
2nd locus specific primer 70wa: 5'-AGATTCTAGAAATGCAAAGACGGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11