Genotyping and primer details


Genotyping and primer details for 668G11 - At3g23760
Line ID 668G11
Locus At3g23760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:8563342 of line 668G11 Chr3:8563342
   
Insertion allele amplicon 8474 - 57gp
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 57gp: 5'-GAAATACAGCGAAAAGAAACTTGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:8563847
   
wt amplicon (over InsPos) 30hs - 57gp
wt amplicon start Chr3:8562869
wt amplicon end Chr3:8563847
wt amplicon size 979 bp
Locus specific primer 57gp: 5'-GAAATACAGCGAAAAGAAACTTGG-3'
2nd locus specific primer 30hs: 5'-TGATTAAAAACGAAAATGACCTCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11