Genotyping and primer details


Genotyping and primer details for 669B05 - At5g08650
Line ID 669B05
Locus At5g08650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:2809801 of line 669B05 Chr5:2809801
   
Insertion allele amplicon 8474 - 03ke
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 03ke: 5'-GGATAAAAACCAAGTTGAGGACAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:2809220
   
wt amplicon (over InsPos) 06kp - 03ke
wt amplicon start Chr5:2809220
wt amplicon end Chr5:2810320
wt amplicon size 1101 bp
Locus specific primer 03ke: 5'-GGATAAAAACCAAGTTGAGGACAA-3'
2nd locus specific primer 06kp: 5'-AGTCGGAGTTCTATCTCCAAATCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11