Genotyping and primer details


Genotyping and primer details for 670B01 - At1g72840
Line ID 670B01
Locus At1g72840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:27412586 of line 670B01 Chr1:27412586
   
Insertion allele amplicon 8474 - 15di
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 15di: 5'-ATATGTTGACGAGACACTTTGCTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:27411939
   
wt amplicon (over InsPos) 63dy - 15di
wt amplicon start Chr1:27411939
wt amplicon end Chr1:27412866
wt amplicon size 928 bp
Locus specific primer 15di: 5'-ATATGTTGACGAGACACTTTGCTG-3'
2nd locus specific primer 63dy: 5'-GATATGATAGCCGAGGTAGTTGGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11