Genotyping and primer details


Genotyping and primer details for 679F10 - At5g37350
Line ID 679F10
Locus At5g37350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:14793549 of line 679F10 Chr5:14793549
   
Insertion allele amplicon 8474 - 39ve
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 39ve: 5'-CTTGATTGGCTAGACGGTAAAGAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:14793163
   
wt amplicon (over InsPos) 43vx - 39ve
wt amplicon start Chr5:14793163
wt amplicon end Chr5:14794244
wt amplicon size 1082 bp
Locus specific primer 39ve: 5'-CTTGATTGGCTAGACGGTAAAGAC-3'
2nd locus specific primer 43vx: 5'-CCCTACAATACATTAACAAGATTCCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11