Genotyping and primer details


Genotyping and primer details for 679F11 - At5g49020
Line ID 679F11
Locus At5g49020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:19873801 of line 679F11 Chr5:19873801
   
Insertion allele amplicon 8474 - 43uv
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 43uv: 5'-AAAGCAAAATTGACCTGAGAGTGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:19874251
   
wt amplicon (over InsPos) 48vl - 43uv
wt amplicon start Chr5:19873433
wt amplicon end Chr5:19874251
wt amplicon size 819 bp
Locus specific primer 43uv: 5'-AAAGCAAAATTGACCTGAGAGTGT-3'
2nd locus specific primer 48vl: 5'-TACCTCTCTATGCAGGCTTTGTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11