Genotyping and primer details


Genotyping and primer details for 681G12 - At1g14600
Line ID 681G12
Locus At1g14600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:5002186 of line 681G12 Chr1:5002186
   
Insertion allele amplicon 8474 - 52sm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 52sm: 5'-CCAGAATCACTCTTCTTGGAAAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:5002816
   
wt amplicon (over InsPos) 11tx - 52sm
wt amplicon start Chr1:5001862
wt amplicon end Chr1:5002816
wt amplicon size 955 bp
Locus specific primer 52sm: 5'-CCAGAATCACTCTTCTTGGAAAAC-3'
2nd locus specific primer 11tx: 5'-GTGTTGACCCATACCATAACAATG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11