Genotyping and primer details


Genotyping and primer details for 686E02 - At2g18100
Line ID 686E02
Locus At2g18100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:7869728 of line 686E02 Chr2:7869728
   
Insertion allele amplicon 8474 - 41kh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 41kh: 5'-CATTGCAAAAGTCAAGTGGAAATA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:7869119
   
wt amplicon (over InsPos) 01ky - 41kh
wt amplicon start Chr2:7869119
wt amplicon end Chr2:7870163
wt amplicon size 1045 bp
Locus specific primer 41kh: 5'-CATTGCAAAAGTCAAGTGGAAATA-3'
2nd locus specific primer 01ky: 5'-TGCAACAAGTTCATGTCAATGTTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11