Genotyping and primer details


Genotyping and primer details for 686F11 - At1g14970
Line ID 686F11
Locus At1g14970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:5164719 of line 686F11 Chr1:5164719
   
Insertion allele amplicon 8474 - BK68
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BK68: 5'-TTTGACAAGTTTGCCAGACAATAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:5164030
   
Insertion allele amplicon 8474 - BK69
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BK69: 5'-GCATTGAATTCTCACGTTTAACAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:5165244
   
wt amplicon (over InsPos) BK69 - BK68
wt amplicon start Chr1:5164030
wt amplicon end Chr1:5165244
wt amplicon size 1215 bp
Locus specific primer BK68: 5'-TTTGACAAGTTTGCCAGACAATAG-3'
2nd locus specific primer BK69: 5'-GCATTGAATTCTCACGTTTAACAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11