Genotyping and primer details


Genotyping and primer details for 692H03 - At2g45720
Line ID 692H03
Locus At2g45720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:18834766 of line 692H03 Chr2:18834766
   
Insertion allele amplicon 8474 - 32sf
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 32sf: 5'-CATCAAGGCATATTTGATCCTTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:18834338
   
wt amplicon (over InsPos) 31tf - 32sf
wt amplicon start Chr2:18834338
wt amplicon end Chr2:18835223
wt amplicon size 886 bp
Locus specific primer 32sf: 5'-CATCAAGGCATATTTGATCCTTTT-3'
2nd locus specific primer 31tf: 5'-CGCTTTCTCCTTAGCAACAATACT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11