Genotyping and primer details


Genotyping and primer details for 693A12 - At5g43720
Line ID 693A12
Locus At5g43720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:17558840 of line 693A12 Chr5:17558840
   
Insertion allele amplicon 8474 - 13ih
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 13ih: 5'-CTCTCAACTCTTCCTCCTTCTTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:17559295
   
wt amplicon (over InsPos) 14ji - 13ih
wt amplicon start Chr5:17558284
wt amplicon end Chr5:17559295
wt amplicon size 1012 bp
Locus specific primer 13ih: 5'-CTCTCAACTCTTCCTCCTTCTTCA-3'
2nd locus specific primer 14ji: 5'-ACCTTCTAACTCCTTCCCACTAGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11