Genotyping and primer details


Genotyping and primer details for 697F05 - At4g28000
Line ID 697F05
Locus At4g28000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:13928738 of line 697F05 Chr4:13928738
   
Insertion allele amplicon 8474 - 04fx
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 04fx: 5'-ACCTGTAAACGCGAAACAGTTAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:13929168
   
wt amplicon (over InsPos) 03jp - 04fx
wt amplicon start Chr4:13928225
wt amplicon end Chr4:13929168
wt amplicon size 944 bp
Locus specific primer 04fx: 5'-ACCTGTAAACGCGAAACAGTTAGT-3'
2nd locus specific primer 03jp: 5'-ATGGTTTGGAGAAGATGAGAAGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11