Genotyping and primer details


Genotyping and primer details for 702E09 - At1g20880
Line ID 702E09
Locus At1g20880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:7263615 of line 702E09 Chr1:7263615
   
Insertion allele amplicon 8474 - 39cs
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 39cs: 5'-AGGATTATGACCGTGTTAACCTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:7264052
   
wt amplicon (over InsPos) 08dn - 39cs
wt amplicon start Chr1:7263023
wt amplicon end Chr1:7264052
wt amplicon size 1030 bp
Locus specific primer 39cs: 5'-AGGATTATGACCGTGTTAACCTTG-3'
2nd locus specific primer 08dn: 5'-ATGACTTCCTGGATGTGAATAACC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11