Genotyping and primer details


Genotyping and primer details for 708C04 - At4g14365
Line ID 708C04
Locus At4g14365 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:8271692 of line 708C04 Chr4:8271692
   
Insertion allele amplicon 8474 - BH62
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BH62: 5'-TCGTTATTTGTTCATCGGTGTTTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:8271117
   
wt amplicon (over InsPos) 57rd - BH62
wt amplicon start Chr4:8271117
wt amplicon end Chr4:8271951
wt amplicon size 835 bp
Locus specific primer BH62: 5'-TCGTTATTTGTTCATCGGTGTTTA-3'
2nd locus specific primer 57rd: 5'-TCCTCCAAGGTTTATGAAAACATC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11