Genotyping and primer details


Genotyping and primer details for 709D04 - At2g44270
Line ID 709D04
Locus At2g44270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:18299088 of line 709D04 Chr2:18299088
   
Insertion allele amplicon 8474 - HP40
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer HP40: 5'-ATTAGGAGAGTAAATGCCTGCAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:18298582
   
wt amplicon (over InsPos) 01fa - HP40
wt amplicon start Chr2:18298582
wt amplicon end Chr2:18299397
wt amplicon size 816 bp
Locus specific primer HP40: 5'-ATTAGGAGAGTAAATGCCTGCAAA-3'
2nd locus specific primer 01fa: 5'-GTTAAGTGATTGTTTTATGGCGGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11