Genotyping and primer details


Genotyping and primer details for 714B08 - At1g07420
Line ID 714B08
Locus At1g07420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:2279932 of line 714B08 Chr1:2279932
   
Insertion allele amplicon 8474 - CR42
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CR42: 5'-ATTTGGTAGCGCAACATCTAAACT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:2280324
   
wt amplicon (over InsPos) 01pc - CR42
wt amplicon start Chr1:2279658
wt amplicon end Chr1:2280324
wt amplicon size 667 bp
Locus specific primer CR42: 5'-ATTTGGTAGCGCAACATCTAAACT-3'
2nd locus specific primer 01pc: 5'-TTTTCTTCCTCTGTATGGAGGGTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11