Genotyping and primer details


Genotyping and primer details for 714G06 - At2g29880
Line ID 714G06
Locus At2g29880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:12742892 of line 714G06 Chr2:12742892
   
Insertion allele amplicon 8474 - 33vh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 33vh: 5'-TGTCATGGTCAGATCAAGAATGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:12742594
   
wt amplicon (over InsPos) 39vu - 33vh
wt amplicon start Chr2:12742594
wt amplicon end Chr2:12743649
wt amplicon size 1056 bp
Locus specific primer 33vh: 5'-TGTCATGGTCAGATCAAGAATGTT-3'
2nd locus specific primer 39vu: 5'-TGGCATGTGACTATCTTTAGGACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11