Genotyping and primer details


Genotyping and primer details for 721A10 - At1g51170
Line ID 721A10
Locus At1g51170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:18953725 of line 721A10 Chr1:18953725
   
Insertion allele amplicon 8474 - AB65
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AB65: 5'-AAGAGATACTCCACTGTCCCTTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:18953167
   
wt amplicon (over InsPos) AR03 - AB65
wt amplicon start Chr1:18953167
wt amplicon end Chr1:18953895
wt amplicon size 729 bp
Locus specific primer AB65: 5'-AAGAGATACTCCACTGTCCCTTTG-3'
2nd locus specific primer AR03: 5'-GTTATTGGTTAAAGATCCGACGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11