Genotyping and primer details


Genotyping and primer details for 722C11 - At3g55140
Line ID 722C11
Locus At3g55140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:20439836 of line 722C11 Chr3:20439836
   
Insertion allele amplicon 8474 - 55sm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 55sm: 5'-GGTCATGATGTTGATGGTATTCAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:20439336
   
wt amplicon (over InsPos) 29tc - 55sm
wt amplicon start Chr3:20439336
wt amplicon end Chr3:20440161
wt amplicon size 826 bp
Locus specific primer 55sm: 5'-GGTCATGATGTTGATGGTATTCAG-3'
2nd locus specific primer 29tc: 5'-ATTTGCTTGAGAGTTTCTGATGGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11