Genotyping and primer details


Genotyping and primer details for 722H01 - At2g24360
Line ID 722H01
Locus At2g24360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:10365806 of line 722H01 Chr2:10365806
   
Insertion allele amplicon 8474 - 35tg
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 35tg: 5'-TGCTGAGATGAGAAGGTTATCTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:10365278
   
wt amplicon (over InsPos) 12tx - 35tg
wt amplicon start Chr2:10365278
wt amplicon end Chr2:10366366
wt amplicon size 1089 bp
Locus specific primer 35tg: 5'-TGCTGAGATGAGAAGGTTATCTGA-3'
2nd locus specific primer 12tx: 5'-TAGCTCGTTTGTCTTAATTTGGGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11