Genotyping and primer details


Genotyping and primer details for 742A03 - At5g39220
Line ID 742A03
Locus At5g39220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:15706919 of line 742A03 Chr5:15706919
   
Insertion allele amplicon 8474 - 33sf
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 33sf: 5'-GGAATTCTTTTGGGCTTTTAGTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:15706361
   
wt amplicon (over InsPos) 30tf - 33sf
wt amplicon start Chr5:15706361
wt amplicon end Chr5:15707487
wt amplicon size 1127 bp
Locus specific primer 33sf: 5'-GGAATTCTTTTGGGCTTTTAGTTC-3'
2nd locus specific primer 30tf: 5'-TCCAATCTATGTTCTCGGATAAGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11