Genotyping and primer details


Genotyping and primer details for 769C10 - At5g54840
Line ID 769C10
Locus At5g54840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:22277193 of line 769C10 Chr5:22277193
   
Insertion allele amplicon 8474 - 05iy
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 05iy: 5'-AATCGTAGTCGATAATGGGTTCAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:22276616
   
wt amplicon (over InsPos) 02jz - 05iy
wt amplicon start Chr5:22276616
wt amplicon end Chr5:22277617
wt amplicon size 1002 bp
Locus specific primer 05iy: 5'-AATCGTAGTCGATAATGGGTTCAC-3'
2nd locus specific primer 02jz: 5'-TACCAATTGCTTGTAAAGATGCAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11