Genotyping and primer details


Genotyping and primer details for 770D07 - At1g77890
Line ID 770D07
Locus At1g77890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:29295352 of line 770D07 Chr1:29295352
   
Insertion allele amplicon 8474 - 41op
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 41op: 5'-GTCACCATATTTACTCCATTGCCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:29295713
   
wt amplicon (over InsPos) 50wn - 41op
wt amplicon start Chr1:29294514
wt amplicon end Chr1:29295713
wt amplicon size 1200 bp
Locus specific primer 41op: 5'-GTCACCATATTTACTCCATTGCCT-3'
2nd locus specific primer 50wn: 5'-CAGGAACTGTTTGTGTCTCCATTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11