Genotyping and primer details


Genotyping and primer details for 771C01 - At4g05520
Line ID 771C01
Locus At4g05520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:2807240 of line 771C01 Chr4:2807240
   
Insertion allele amplicon 8474 - 25vd
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 25vd: 5'-CCGCTTTTTCTAAAGCTAATGAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:2806791
   
wt amplicon (over InsPos) 72vo - 25vd
wt amplicon start Chr4:2806791
wt amplicon end Chr4:2807698
wt amplicon size 908 bp
Locus specific primer 25vd: 5'-CCGCTTTTTCTAAAGCTAATGAGA-3'
2nd locus specific primer 72vo: 5'-AAAATGTTCCACACTTGGAAAGTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11