Genotyping and primer details


Genotyping and primer details for 779D08 - At1g61030
Line ID 779D08
Locus At1g61030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:22481542 of line 779D08 Chr1:22481542
   
Insertion allele amplicon 8474 - 75ra
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 75ra: 5'-ACGGAATAAACAGGTACCAAACTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:22481217
   
wt amplicon (over InsPos) 13rt - 75ra
wt amplicon start Chr1:22481217
wt amplicon end Chr1:22481949
wt amplicon size 733 bp
Locus specific primer 75ra: 5'-ACGGAATAAACAGGTACCAAACTG-3'
2nd locus specific primer 13rt: 5'-CGCATACCACATCGACTACTAAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11