Genotyping and primer details


Genotyping and primer details for 781E10 - At5g49560
Line ID 781E10
Locus At5g49560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:20111196 of line 781E10 Chr5:20111196
   
Insertion allele amplicon 8474 - 19hg
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 19hg: 5'-TCAAAGTCTGTGTGACTGTGTGTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:20110622
   
wt amplicon (over InsPos) 14ig - 19hg
wt amplicon start Chr5:20110622
wt amplicon end Chr5:20111739
wt amplicon size 1118 bp
Locus specific primer 19hg: 5'-TCAAAGTCTGTGTGACTGTGTGTC-3'
2nd locus specific primer 14ig: 5'-ACGAAGCTAAAATGCTCCTCATAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11