Genotyping and primer details


Genotyping and primer details for 785A01 - At1g78100
Line ID 785A01
Locus At1g78100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:29387932 of line 785A01 Chr1:29387932
   
Insertion allele amplicon 8474 - MN41
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MN41: 5'-TTAGCTGGTTTGGAGAAGATAGGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:29388175
   
wt amplicon (over InsPos) 21vb - MN41
wt amplicon start Chr1:29387662
wt amplicon end Chr1:29388175
wt amplicon size 514 bp
Locus specific primer MN41: 5'-TTAGCTGGTTTGGAGAAGATAGGA-3'
2nd locus specific primer 21vb: 5'-CAATACAATATTCGAAAAAGCCGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11