Genotyping and primer details


Genotyping and primer details for 785F02 - At2g20100
Line ID 785F02
Locus At2g20100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:8680998 of line 785F02 Chr2:8680998
   
Insertion allele amplicon 8474 - 25wm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 25wm: 5'-TGTGATCTAGTGGGAGTATTGGTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:8681552
   
wt amplicon (over InsPos) 26xg - 25wm
wt amplicon start Chr2:8680000
wt amplicon end Chr2:8681552
wt amplicon size 1553 bp
Locus specific primer 25wm: 5'-TGTGATCTAGTGGGAGTATTGGTG-3'
2nd locus specific primer 26xg: 5'-TCTCTTCACCAGCTAGTATCTCCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11