Genotyping and primer details


Genotyping and primer details for 787G02 - At5g57480
Line ID 787G02
Locus At5g57480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:23279497 of line 787G02 Chr5:23279497
   
Insertion allele amplicon 8474 - 31ew
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 31ew: 5'-TCCCCACATAGAAAAATCTTCATC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:23279129
   
wt amplicon (over InsPos) 07fq - 31ew
wt amplicon start Chr5:23279129
wt amplicon end Chr5:23279792
wt amplicon size 664 bp
Locus specific primer 31ew: 5'-TCCCCACATAGAAAAATCTTCATC-3'
2nd locus specific primer 07fq: 5'-ATGAGTTTTTGTAACTTCCCATCG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11