Genotyping and primer details


Genotyping and primer details for 791G05 - At1g15270
Line ID 791G05
Locus At1g15270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:5250905 of line 791G05 Chr1:5250905
   
Insertion allele amplicon 8474 - 01dp
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 01dp: 5'-TTTGATGCTCTGACTTGTTTTCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:5251437
   
wt amplicon (over InsPos) 76dy - 01dp
wt amplicon start Chr1:5250494
wt amplicon end Chr1:5251437
wt amplicon size 944 bp
Locus specific primer 01dp: 5'-TTTGATGCTCTGACTTGTTTTCTC-3'
2nd locus specific primer 76dy: 5'-CCCTCAGTTTCTACCTCATCTTGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11