Genotyping and primer details


Genotyping and primer details for 794E12 - At3g15810
Line ID 794E12
Locus At3g15810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:5348467 of line 794E12 Chr3:5348467
   
Insertion allele amplicon 8474 - 40vf
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 40vf: 5'-GGTGATATCATCTTCAGGGTTGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:5349019
   
wt amplicon (over InsPos) 79vo - 40vf
wt amplicon start Chr3:5348322
wt amplicon end Chr3:5349019
wt amplicon size 698 bp
Locus specific primer 40vf: 5'-GGTGATATCATCTTCAGGGTTGAT-3'
2nd locus specific primer 79vo: 5'-CCGTCGTAAACTTCTACTTCCATC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11