Genotyping and primer details


Genotyping and primer details for 796F06 - At1g12000
Line ID 796F06
Locus At1g12000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:4052591 of line 796F06 Chr1:4052591
   
Insertion allele amplicon 8474 - 35ve
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 35ve: 5'-AATAGGGCACAATAGAAATGATGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:4051995
   
wt amplicon (over InsPos) 37vx - 35ve
wt amplicon start Chr1:4051995
wt amplicon end Chr1:4053396
wt amplicon size 1402 bp
Locus specific primer 35ve: 5'-AATAGGGCACAATAGAAATGATGC-3'
2nd locus specific primer 37vx: 5'-TCTCGTGTAGTTTGAGAATGGATG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11