Genotyping and primer details


Genotyping and primer details for 803D09 - At3g16110
Line ID 803D09
Locus At3g16110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:5462224 of line 803D09 Chr3:5462224
   
Insertion allele amplicon 8474 - 62gp
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 62gp: 5'-AATGCAGCAACCTGTTAAAGAACT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:5461876
   
wt amplicon (over InsPos) 02ig - 62gp
wt amplicon start Chr3:5461876
wt amplicon end Chr3:5462715
wt amplicon size 840 bp
Locus specific primer 62gp: 5'-AATGCAGCAACCTGTTAAAGAACT-3'
2nd locus specific primer 02ig: 5'-TGGTTAAAACTGAGGCTGAAAAGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11