Genotyping and primer details


Genotyping and primer details for 805C04 - At4g22430
Line ID 805C04
Locus At4g22430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:11828065 of line 805C04 Chr4:11828065
   
Insertion allele amplicon 8474 - GA50
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer GA50: 5'-ATGGGTGCATAACATCCAATAAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:11828583
   
wt amplicon (over InsPos) 47rd - GA50
wt amplicon start Chr4:11827684
wt amplicon end Chr4:11828583
wt amplicon size 900 bp
Locus specific primer GA50: 5'-ATGGGTGCATAACATCCAATAAAT-3'
2nd locus specific primer 47rd: 5'-CATCACAGATGTTTTAGAGGGGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11