Genotyping and primer details


Genotyping and primer details for 810E08 - At3g20350
Line ID 810E08
Locus At3g20350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:7097171 of line 810E08 Chr3:7097171
   
Insertion allele amplicon 8474 - 34qn
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 34qn: 5'-GACTCAGACAAAGGAATAGCCAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:7097781
   
wt amplicon (over InsPos) 42sq - 34qn
wt amplicon start Chr3:7096881
wt amplicon end Chr3:7097781
wt amplicon size 901 bp
Locus specific primer 34qn: 5'-GACTCAGACAAAGGAATAGCCAAC-3'
2nd locus specific primer 42sq: 5'-GATACTGTTAGGAAACTTGCTGCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11