Genotyping and primer details


Genotyping and primer details for 812G08 - At5g09840
Line ID 812G08
Locus At5g09840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:3061689 of line 812G08 Chr5:3061689
   
Insertion allele amplicon 8474 - 51kh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 51kh: 5'-AACCCCATCTTCTTCTTTACCATT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:3061199
   
wt amplicon (over InsPos) 04li - 51kh
wt amplicon start Chr5:3061199
wt amplicon end Chr5:3062064
wt amplicon size 866 bp
Locus specific primer 51kh: 5'-AACCCCATCTTCTTCTTTACCATT-3'
2nd locus specific primer 04li: 5'-GTTTACCATAGACCAAACCCAAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11