Genotyping and primer details


Genotyping and primer details for 821H10 - At2g37840
Line ID 821H10
Locus At2g37840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:15852170 of line 821H10 Chr2:15852170
   
Insertion allele amplicon 8474 - FQ50
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer FQ50: 5'-CCCGATCTTATCATATAAGCCAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:15851746
   
Insertion allele amplicon 8474 - JL35
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer JL35: 5'-TTTACCTAACTGCTGCATGAAATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:15852584
   
wt amplicon (over InsPos) FQ50 - JL35
wt amplicon start Chr2:15851746
wt amplicon end Chr2:15852584
wt amplicon size 839 bp
Locus specific primer JL35: 5'-TTTACCTAACTGCTGCATGAAATG-3'
2nd locus specific primer FQ50: 5'-CCCGATCTTATCATATAAGCCAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11